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	Provedor de dados BABT (1) Genet. Mol. Biol. (1) Autor Leão,Mariele Porto Carneiro (2) Andreote,Fernando Dini (1) Camargo,Luis Eduardo Aranha (1) Maciel,Danielli Barreto (1) Medeiros,Lílian Vieira de (1) Medeiros,Vivian Vieira de (1) Oliveira,Neiva Tinti de (1) Tiago,Patricia Vieira (1) de Araújo,Welington Luiz (1) de Oliveira,Neiva Tinti (1) Mais... Palavra-chave Cap20 gene (1) Colletotrichum gloeosporioides (1) Diatraea saccharalis (1) Entomopathogen (1) Expression pattern (1) Pathogenicity (1) Quantitative RT-PCR (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2015 (1) 2010 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Amplification of the cap20 pathogenicity gene and genetic characterization using different markers molecular in Colletotrichum gloeosporioides isolates BABT Maciel,Danielli Barreto; Medeiros,Lílian Vieira de; Medeiros,Vivian Vieira de; Leão,Mariele Porto Carneiro; Camargo,Luis Eduardo Aranha; Oliveira,Neiva Tinti de. Studies were performed to analyze the genetic characterization using RFLP-ITS and Intron (primer EI1) markers and the amplification of the cap20 pathogenicity gene by PCR in Colletotrichum gloeosporioides isolates of different hosts plant. The genetic variability was accessed using RFLP-ITS and Intron markers and grouping by UPGMA method. Primers to cap20 gene were constructed using selected sequences of the GenBank (National Center of Biotechnology Information, http://www.ncbi.nlm.nih.gov) with the Primer 3 program. The dendrograms analysis showed that the RFLP-ITS marker was more informative to separate the Colletotrichum sp, and that primer EI1 demonstrated greater genetic diversity. The amplification of the DNA of the Colletotrichum isolates to the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cap20 gene; Colletotrichum gloeosporioides; Pathogenicity. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600001 Differential expression of the pr1A gene in Metarhizium anisopliae and Metarhizium acridum across different culture conditions and during pathogenesis Genet. Mol. Biol. Leão,Mariele Porto Carneiro; Tiago,Patricia Vieira; Andreote,Fernando Dini; de Araújo,Welington Luiz; de Oliveira,Neiva Tinti. The entomopathogenic fungi of the genus Metarhizium have several subtilisin-like proteases that are involved in pathogenesis and these have been used to investigate genes that are differentially expressed in response to different growth conditions. The identification and characterization of these proteases can provide insight into how the fungus is capable of infecting a wide variety of insects and adapt to different substrates. In addition, the pr1A gene has been used for the genetic improvement of strains used in pest control. In this study we used quantitative RT-PCR to assess the relative expression levels of the pr1A gene in M. anisopliae and M. acridum during growth in different culture conditions and during infection of the sugar cane borer,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Entomopathogen; Diatraea saccharalis; Quantitative RT-PCR; Expression pattern. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100086 Registros recuperados: 2 Primeira ... 1 ... Última

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